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customized microarray chip  (Agilent technologies)


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    Structured Review

    Agilent technologies customized microarray chip
    Customized Microarray Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customized+microarray+chip/pm38158220-248-32-41
    Average 90 stars, based on 1 article reviews
    customized microarray chip - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies
    Article Snippet: 4 μg of SMART-PCR amplified products were labelled with fluorescent dye Cy5 (Amersham) and mixed with equal amount of Cy3-labelled P. falciparum 3D7 reference pool comprising of equal mass of total RNA samples representing all developmental stages of the parasite [ ]. .. Hybridization was carried at 65 °C for 20 h on our customized microarray chip [ ] using the Agilent hybridization system. .. Scanning and image acquisition of the hybridized microarray chips was performed using the PowerScannerTM (Tecan) at 10 μM resolution under automated photomultiplier (PMT) balancing.

    Article Title: Artemisinin resistance in the malaria parasite, Plasmodium falciparum, originates from its initial transcriptional response.
    Article Snippet: The amplified product was purified using Ampure XP magnetic beads (Beckman Coulter) on Microlab Nimbus robotic platform (Hamilton) and 100 ng of cDNA was used for subsequent 10 rounds of amplification to generate aminoallyl-coupled cDNA for the hybridizations74,75. .. 17ul (~5 μg) of each Cy-5labelled (GE Healthcare) cDNA of patient’s sample and an equal amount of Cy-3labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on our customized microarray chip using commercially available hybridization platform (Agilent) for 20 h at 70 °C with rotation at 10 rpm. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10 μM resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Microarray:

    Article Title: Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies
    Article Snippet: 4 μg of SMART-PCR amplified products were labelled with fluorescent dye Cy5 (Amersham) and mixed with equal amount of Cy3-labelled P. falciparum 3D7 reference pool comprising of equal mass of total RNA samples representing all developmental stages of the parasite [ ]. .. Hybridization was carried at 65 °C for 20 h on our customized microarray chip [ ] using the Agilent hybridization system. .. Scanning and image acquisition of the hybridized microarray chips was performed using the PowerScannerTM (Tecan) at 10 μM resolution under automated photomultiplier (PMT) balancing.

    Article Title: Artemisinin resistance in the malaria parasite, Plasmodium falciparum, originates from its initial transcriptional response.
    Article Snippet: The amplified product was purified using Ampure XP magnetic beads (Beckman Coulter) on Microlab Nimbus robotic platform (Hamilton) and 100 ng of cDNA was used for subsequent 10 rounds of amplification to generate aminoallyl-coupled cDNA for the hybridizations74,75. .. 17ul (~5 μg) of each Cy-5labelled (GE Healthcare) cDNA of patient’s sample and an equal amount of Cy-3labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on our customized microarray chip using commercially available hybridization platform (Agilent) for 20 h at 70 °C with rotation at 10 rpm. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10 μM resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Chromatin Immunoprecipitation:

    Article Title: Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies
    Article Snippet: 4 μg of SMART-PCR amplified products were labelled with fluorescent dye Cy5 (Amersham) and mixed with equal amount of Cy3-labelled P. falciparum 3D7 reference pool comprising of equal mass of total RNA samples representing all developmental stages of the parasite [ ]. .. Hybridization was carried at 65 °C for 20 h on our customized microarray chip [ ] using the Agilent hybridization system. .. Scanning and image acquisition of the hybridized microarray chips was performed using the PowerScannerTM (Tecan) at 10 μM resolution under automated photomultiplier (PMT) balancing.

    Gentle:

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.



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